A Guided Tour of the Structural Biology of Gaucher Disease: Acid-β-Glucosidase and Saposin C

Table 1

Crystal structures reported for GCase.

PDB code	Enzyme source	Deglycosylated?	Active site	Crystallization condition	pH	Ref.
1OGS	CHO/Cerezyme	PD^a	Sulfate	Ammonium sulfate, Guanidinium HCl, KCl, acetate buffer, cryoprotected with glycerol	4.6	[24]
1Y7V	CHO/Cerezyme	PD	CBE	Same as 1OGS, soaking overnight with 1 mM CBE, cryoprotected with glycerol	4.6	[33]
2F61	CHO/Cerezyme	PD		Ammonium sulfate, citrate buffer, magnesium chloride	6	[16]
2J25	CHO/Cerezyme	No		Ammonium sulfate, bis-tris buffer	5.5	[34]
2NSX	CHO/Cerezyme	PD	IFG	Same as 1OGS, soaking for 10′ with 0.2 mM IFG	4.5	[35]
2NT0	CHO/Cerezyme	PD	Glycerol	Same as 1OGS	4.5	[35]
2NT1	CHO/Cerezyme	PD		Na, K Dihydrogen phosphate, Hepes buffer, lithium sulfate cryoprotectant	7.5	[35]
2V3D	Plant/Taliglucerase-alfa	No	NB-DNJ	Ammonium sulfate, Tris buffer, PEG 3350; cocrystallization with ligand	6.5	[36]
2V3E	Plant/Taliglucerase-alfa	No	NN-DNJ	Ammonium acetate, Hepes buffer, PEG 3350; cocrystallization with ligand	7.5	[36]
2V3F	Plant/Taliglucerase-alfa	No	N/A	Ammonium sulfate, bis-Tris buffer, hexamine cobalt(III) chloride, PEG 3350	6.5	[29]
2VT0	Plant^1	N/A^b	N/A	Ammonium sulfate, Tris buffer, PEG 3350	6.5	N/A
2WCG	Plant/Taliglucerase-alfa	No	N-octyl(cyclic guanidine)-nojirimycin	Same as 2V3D, cocrystallization with ligand	6.5	[37]
2WKL	Human cell line/Velagucerase-alfa	No		Ammonium sulfate, Hepes buffer, PEG 8000, ethylene glycol cryoprotectant	7	[30]
3GXD	CHO/Cerezyme	PD		Na, K Dihydrogen phosphate, acetate buffer, lithium sulfate cryoprotectant	4.5	[38]
3GXF	CHO/Cerezyme	PD	IFG	Na, K Dihydrogen phosphate, Hepes buffer, glycerol cryoprotectant, soaking for 10′ with 0.5 mM IFG	7.5	[38]
3GXI	CHO/Cerezyme	PD		Na, K Dihydrogen phosphate, citrate buffer, lithium sulfate cryoprotectant	5.5	[38]
3GXM	CHO/Cerezyme	PD		Same as 1OGS	4.5	[38]
3KE0	Baculovirus (N370S-GCase)	PD		Same as 1OGS	5.4	[32]
3KEH	Baculovirus (N370S-GCase)	PD		Na, K Dihydrogen phosphate, Hepes buffer, glycerol cryoprotectant	7.4	[32]

^aPD: partially glycosylated
^bN/A: Not applicable or not available.