A Thermostable Crude Endoglucanase Produced by Aspergillus fumigatus in a Novel Solid State Fermentation Process Using Isolated Free Water

Table 2

Kinetic and thermodynamic parameters of irreversible thermal denaturation of crude endoglucanase from A. fumigatus grown for 7 days under SSF_H2O conditions.

Temperature		𝑘𝑑^$ (min^−1)	T1/2 (min)	Δ𝐻^∗𝐷 (K J mol^−1)	Δ𝐺^∗𝐷 (K J mol^−1)	Δ𝑆^∗𝐷 (J mol^−1 K^−1)
^°C	K
60	333	0.0001	6930	254.04	118.69	406.45
70	343	0.0008	866	253.96	116.41	401.01
80	353	0.0194	36	253.88	110.53	406.07

^$: Values of 𝑘_𝑑 were obtained from Figure 3.
Note: Activation energy of denaturation (𝐸_𝑎(𝐷)) used to estimate Δ𝐻^∗_𝐷 was calculated using equation: 𝐸_𝑎(𝐷) = −(slope × 𝑅) = 256.811 K J mole⁻¹ where the value of slope was obtained from Figure 4.