Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

Table 4

pH stability of the free and immobilized lipase.*

pH	Relative activity (%)
	Free lipase (FLCS)	Immobilized lipase (IE)
Control**	100^g,h,i	100^g,h,i
3.0	155 ± 0^e	100 ± 6.0^g,h,i
4.0	52 ± 9.2^l,m,n	69 ± 13.1^j,k,l,m
5.0	83 ± 2.1^h,i,j,k,l	239 ± 10.1^c
6.0	70 ± 11.6^i,j,k,l,m	97 ± 10.8^g,h,i,j
7.0	117 ± 13.7^f,g	150 ± 0.1^e
8.0	99 ± 9.2^g,h,i,j	143 ± 5.9^e,f
9.0	39 ± 10.9^m,n	97 ± 7.4^g,h,i,j

*The free and immobilized enzymes were incubated at different buffer solutions for 2 h at 50°C.
** Each pH studied had a different control. Control: free and immobilized lipase with respective buffer solution analyzed without incubation.
***Mean values with the same letter do not statistically differ from each other by the ANOVA Tukey test (𝑃=0.01).