Optimal Conditions for Continuous Immobilization of Pseudozyma hubeiensis (Strain HB85A) Lipase by Adsorption in a Packed-Bed Reactor by Response Surface Methodology

Table 5

Effect of diverse chemicals and detergents on P. hubeiensis free and immobilized lipase activity.*

Substance	Concentration	Relative activity (%)***
		Free lipase (FLCS)	Immobilized lipase (IE)
Control**		100	100
MgCl2	5 mM	98±11.4^c,d	144±3.6^b
KCl	5 mM	65±8.8^d,e,f	37±6.2^f,g
BaCl2	5 mM	7±2.8^g	100±3.8^c
CaCl2	5 mM	185±11.4^a	58±4.2^e,f
ZnSO4	5 mM	85±4.0^c,d,e	98±3.6^c,d
EDTA	5 mM	55±0^e,f	103±7.7^c
β-mercaptoethanol	5 mM	62±4.4^e,f	139±11.7^b
Triton X-100	1%	107±12.4^d	330±13.6^a
Tween 20	1%	123±0^c,d	250±3.0^b
Tween 80	1%	103±10.3^d	149±6.8^c
SDS	1%	0^e	0^e

*The free and immobilized enzymes were incubated in the presence of various compounds at 50°C for 1 h.
**Control: free and immobilized lipase without the addition of any substance.
***Mean values with the same letter do not statistically differ from each other by the ANOVA Tukey test (𝑃=0.01).