The Effect of D-(−)-arabinose on Tyrosinase: An Integrated Study Using Computational Simulation and Inhibition Kinetics

<table>Plots of enzyme activity (<svg height="7.9499998" id="M33" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 8.2875004 7.9499998" width="8.2875004" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M457 332q0 -81 -41 -161.5t-105.5 -131.5t-129.5 -51q-46 0 -79 30.5t-33 86.5q0 18 7 51q18 95 46 187q6 19 6 33q0 7 -7 7q-24 0 -78 -64l-20 23q32 48 73 77t78 29q33 0 33 -46q0 -38 -10 -70q-28 -92 -39 -152q-7 -38 -7 -57q0 -78 69 -78q68 0 118 75.5t50 194.5
q0 43 -12 57q-11 12 -11 24q0 19 15 35.5t34 16.5q18 0 30.5 -34.5t12.5 -81.5z" id="x1D463"></path></g>
</svg>) versus enzyme concentration [E] at D-(–)-arabinose concentrations of 0 M (■), 0.2 M (●), 0.6 M (▲), and 0.8 M (<i>▼</i>). The <svg height="7.9499998" id="M34" style="vertical-align:-0.1638pt" version="1.1" viewbox="0 0 9.1625004 7.9499998" width="9.1625004" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,.062,7.675)"><path d="M509 373q0 -52 -42.5 -118.5t-124.5 -159.5l-94 -107h-27q-22 209 -78 342q-21 48 -41 48q-18 0 -63 -62l-22 21q77 111 125 111q30 0 52 -21.5t38 -75.5q37 -130 52 -285q48 62 74.5 99.5t53.5 87t27 76.5q0 43 -17 60q-5 5 -5 15q0 17 12.5 30.5t28.5 13.5
q21 0 36 -22.5t15 -52.5z" id="x1D708"></path></g>
</svg> value indicates the change in absorbance at 475 nm per minute. The final L-DOPA concentration was 2 mM.</table>

Enzyme Research

fig5

Figure 5

Figure 5: The Effect of D-(−)-arabinose on Tyrosinase: An Integrated Study Using Computational Simulation and Inhibition Kinetics 