Use of Fluorochrome-Labeled Inhibitors of Caspases to Detect Neuronal Apoptosis in the Whole-Mounted Lamprey Brain after Spinal Cord Injury

Figure 4

Photomicrographs of dorsal views of whole-mounted brains of larval sea lampreys show activated caspases in identified reticulospinal neurons after a complete SC transection as revealed by FAM-LETD-FMK labeling (green channel). (a) Photomicrograph of the rhombencephalon of a larval sea lamprey shows FAM-LETD-FMK labeling in the Mth, B1, B3, and B4 neurons 2 weeks after a complete SC transection. Note also a nonlabeled B5 cell (arrow). (b) Photomicrograph of the rostral rhombencephalon of a larval sea lamprey shows FAM-LETD-FMK labeling in the I1 and I2 neurons 2 weeks after a complete SC transection. (c) Photomicrograph of the mesencephalon/diencephalon of a larval sea lamprey shows FAM-LETD-FMK labeling in the M3 and M2 neurons 2 weeks after a complete SC transection. (d) Photomicrograph of the mesencephalon/diencephalon of a larval sea lamprey shows FAM-LETD-FMK labeling in the M1, M2, and M3 neurons 2 weeks after a complete SC transection. Rostral is up in all figures. The ventricle is at the right in all figures. For abbreviations, see list.