Statistical Approach for Optimization of Physiochemical Requirements on Alkaline Protease Production from Bacillus licheniformis NCIM 2042

Table 3

Estimated effect and analysis of variables for protease activity from Plackett-Burman design experiment.

Factor	Name	Studentized effect	Sum square	Percentage of contribution	Standard error	𝑡-value	P value
𝐴	Inoculum percentage (%)	15.92	760.29	2.72	0.34	47.44	<0.0001
𝐵	Temperature (°C)	29.35	2585.08	9.26	0.34	87.48	<0.0001
𝐶	pH	27.49	2267.32	8.12	0.34	81.93	<0.0001
𝐷	Agitation (RPM)	82.11	20228.37	72.46	0.34	244.71	<0.0001
𝐸	Incubation time (h)	26.25	2067.06	7.40	0.34	78.23	<0.0001