Pullulanase: Role in Starch Hydrolysis and Potential Industrial Applications

Table 2

Reaction specificities of pullulan-degrading enzymes.

Enzyme	EC number	Bonds processed	Preferred substrate	End products	References
Pullulanase type I	3.2.1.41	α-(1,6)	Oligo- and polysaccharides, Pullulan	Trimer (maltotriose)	[17, 27]
Pullulanase type II; (amylopullulanase)		α-(1,6)	Pullulan,	Trimer (maltotriose)
	3.2.1.41	α-(1,4)	Poly- and oligosaccharide (starch)	Mixture of glucose, maltose, and maltotriose	[31, 32, 40]
Pullulan hydrolase type I; (neopullulanase)	3.2.1.135	α-(1,4)	Pullulan	Panose	[41–43]
Pullulan hydrolase type II; (isopullulanase)	3.2.1.57	α-(1,4)	Pullulan	Isopanose	[12]
Pullulan hydrolase type III		α-(1,4) and; α-(1,6)	Pullulan	Mixture of panose, maltose, and maltotriose
	3.2.1.—		Starch, amylose, and amylopectin	Maltotriose and maltose	[37]