Pullulanase: Role in Starch Hydrolysis and Potential Industrial Applications

Table 6

Genetic engineering of pullulanase gene.

Source	Expression strain	Length (a.a)*	Vector	References
Anaerobranca gottschalkii	E. coli BL21 (DE3)	865	pET21a	[60]
Bacillus thermoleovorans US105	E. coli DH5α	718	pEBM2	[30]
Bacillus stearothermophilus TS-23	E. coli XL1-Blue MRF	2018	pAP/StuI	[61]
Bacillus stearothermophilus strain TRS-128	B. subtilis NA-1	N.A.^#	pTB522	[62]
Klebsiella pneumonia	E. coli K-12	N.A.^#	pACYC184	[63]
Thermus IM6501	E. coli	718	p6xHis119	[64]
Desulfurococcus mucosus	B. subtilis clone JA803	686	JA803	[32]
Fervidobacterium pennivorans Ven5	E. coli FD748	849	pSE420	[29]
Thermococcus hydrothermalis	E. coli JM109; (DE)	1339	pAPUΔ2	[65]
Thermotoga maritima	E. coli JM83	840	pTPU1	[66]
Caldicellulosiruptor saccharolyticus	E. coli Q359	826	pNZ1038	[67]
Bacillus sp. XAL601 II	E. coli JM109	2032	pUC18	[68]

*a.a: amino acid.
^#N.A.: not available.