Research Article
An Acidic Thermostable Recombinant Aspergillus nidulans Endoglucanase Is Active towards Distinct Agriculture Residues
Table 1
Purification of the recombinant endoglucanase from the supernatant of P. pastoris.
| | Fractions | Total protein (µg) | Recombinant endoglucanase A activity | | Total activity (IU) | Specific activity (IU/mg) | Yield (%) | Purification fold (x) |
| | Crude extract | 36.69 | 116.94 | 3.2 | 100 | 1 | | Concentrated fraction (MW50) | 19.97 | 9.45 | 0.5 | ND | ND | | Ultrafiltered fraction (MW50) | 21.35 | 77.04 | 3.6 | 65.9 | 1.1 | | Concentrated fraction (MW10) | 12.51 | 3.95 | 0.3 | 3.4 | 0.1 | | Ultrafiltered fraction (MW10) | 24.66 | 89.57 | 3.6 | ND | ND | | Sephadex G50 | 0.31 | 4.33 | 14.1 | 3.7 | 4.4 | | Dialyzed | 0.36 | 4.12 | 11.5 | 3.5 | 3.6 | | Q-Sepharose | 0.04 | 2.40 | 55.8 | 2.1 | 17.5 |
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ND: not determined.
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