Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation

Figure 1

Elution of PG activity from chromatography columns previously equilibrated with 20 mM acetate buffer, pH 4.0: (a) Sephadex G-75 column (3.0 × 80 cm) and (b) SP Sepharose (2.5 × 20 cm) eluted with a NaCl gradient (0–1.1 M). -■- PG activity; -□- protein; — NaCl gradient.

(a)

(b)