Research Article
Purification and Properties of Polygalacturonase Produced by Thermophilic Fungus Thermoascus aurantiacus CBMAI-756 on Solid-State Fermentation
Table 1
Purification of the PG produced by Thermoascus aurantiacus in SSF.
| | Step | Volume (mL) | Total activity (U) | Total protein (mg) | Specific activity
(U/mg protein) | Purification fold | Yield (%) |
| | Lyophilized extract | 8.00 | 1800.00 | 30.00 | 60.00 | 1.00 | 100.00 | | Sephadex G-75 | 54.00 | 1058.40 | 3.19 | 331.60 | 5.53 | 58.80 | | SP Sepharose | 12.60 | 255.80 | 0.0478 | 5351.50 | 89.19 | 14.20 |
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