Cloning, Expression, and Purification of Nucleoside Diphosphate Kinase from Acinetobacter baumannii

Figure 4

(a) Effect of increasing urea concentrations on fluorescence intensity of Ndk. (b) Effect of increasing concentrations of ATP on fluorescence intensity of Ndk at 3 different pH conditions: acidic (▼), neutral (●) and basic (■). (c) The binding curve of ATP with Ndk. The quenching 𝑄=(𝐹_𝑜−𝐹)/𝐹_𝑜 in percent was plotted against increasing ATP concentrations.

(a)

(b)

(c)