Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans

Table 2

Yields of the immobilization of NStcI esterase.

	Total protein; (mg)	Total activity; (U)	Specific activity; (U/mg of protein)
Free esterase	9.42±0.38	4335.80±14.22	459.40±0.18
Supernatant	5.42±0.27	783.07±1.12	130.43±0.32
Immobilized esterase	4.00±0.15	3552.80±21.45^∗	412.50±0.63^†

∗This value was obtained by difference between the free esterase solution and supernatant.
^†Activity value was obtained experimentally as described in Section 2.4.