Immobilization and Biochemical Properties of the Enantioselective Recombinant NStcI Esterase of Aspergillus nidulans

Table 3

Effect of solvent and 𝑎_w on the enzymatic resolution of (𝑅/𝑆)-1-phenylethanol by esterase NStcI.

Solvent	𝑎w initial	Reaction time (h)	Enzyme	%𝑒𝑒𝑝	𝐸	%𝐶𝑝
Hexane	0.15	96	NStcI	45.1±5.8	12.3±2.1	31.2±2.7
			Novozym 435	59.4±2.9	19.2±1.8	37.5±1.1
	0.07	120	NStcI	71.7±4.9	31.3±6.9	42.0±1.6
			Novozym 435	85.5±1.9	67.5±9.9	46.3±0.5
Diisopropyl ether	0.07	120	NStcI	0	0	0
			Novozym 435	62.4±2.0	21.3±1.5	38.7±0.7

All results were represented as the mean ± S.D. of 3 separated determinations.