Molecular Cloning and Optimization for High Level Expression of Cold-Adapted Serine Protease from Antarctic Yeast Glaciozyma antarctica PI12

Figure 3

Neighbor-joining phylogenetic analysis of internal transcribed spacer 1 (ITS1)/5.8S rRNA gene/internal transcribed spacer 2 (ITS2) of Glaciozyma clade. Numbers indicate percentage bootstrap values calculated on 1000 repeats of the alignment. The tree was constructed using MEGA 6 software [39]. The scale bar represents 0.01 substitutions per nucleotide position. GenBank accession numbers of the sequences are indicated before strain name. ITS1/5.8S/ITS2 sequence of Glaciozyma antarctica strain PI12 had been deposited in GenBank with the accession number JX896956 [14].