Contemporaneous Production of Amylase and Protease through CCD Response Surface Methodology by Newly Isolated Bacillus megaterium Strain B69

Table 4

Central composite design matrix for the experimental design and predicted responses for protease activity.

Std	Factor 1	Factor 2	Factor 3	Response 1		Response 2
	A: substrate concentration	B: moisture content	C: time	Protease activity (U/g)		Amylase activity (U/g)
	%	%	h	Actual	Predicted	Actual	Predicted
1	10	30	48	246.15	216.05	204.03	153.31
2	30	30	48	625	597.6	296.33	375.19
3	10	60	48	630.19	616.55	480.9	454.95
4	30	60	48	737.69	769.92	560.9	621.95
5	10	30	120	595.58	565.61	661.67	704.32
6	30	30	120	931.15	947.06	920.24	1049.88
7	10	60	120	685.96	715.63	847.57	872.4
8	30	60	120	836.54	868.91	1008.67	1163.09
9	3.18	45	84	403.85	431.13	198.33	253.79
10	36.82	45	84	911.35	880.86	886.9	684.8
11	20	19.77	84	845	888.64	1085.37	1016.18
12	20	70.23	84	1206.54	1159.69	1442.49	1365.02
13	20	45	23.45	194.23	218.46	51.69	64.08
14	20	45	144.55	623.08	595.64	1141.5	982.46
15	20	45	84	1218.46	1219.19	1525.84	1606.93
16	20	45	84	1240.2	1239.21	1462.77	1606.93
17	20	45	84	1242.12	1239.21	1666.31	1606.93
18	20	45	84	1220.05	1239.21	1625.82	1606.93
19	20	45	84	1242.94	1239.21	1643.57	1606.93
20	20	45	84	1251	1239.21	1592.13	1606.93