Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study
Figure 3
Effect of additives on the structure and activity of xylanase. (a) Far-UV CD spectra of 0.09 mg·mL−1 protein (solid line), with 2 M sorbitol (dotted line) and with 0.5% (w/v) soluble xylan (short dashed line) at 20°C. After the T ramp, the spectra of samples with or without additives were recorded at 85°C. (b) Plot of with increasing temperature of xylanase (dotted line), in the presence of 2 M sorbitol (solid line) and 0.5% (w/v) soluble xylan (short dashed line). Data were fitted to (1). (c) Fluorescence emission spectra of 0.09 mg·mL−1 xylanase (dotted line), with 2 M sorbitol (solid line), 0.4 M GL (long dashed line), or 0.5% soluble xylan (short dashed line). Inset: dependence of the fluorescence intensity on sorbitol (void circles) and GL (filled circles) concentration. (d) Effect of sorbitol (void circles) and GL (filled circles) on the activity of xylanase. GL data were fitted to (1).
