Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study

Figure 4

Irreversible thermal unfolding of xylanase followed by DSC. (a) Variation of the excess heat capacity () of 1.1 mg·mL⁻¹ xylanase with the temperature at a scan rate of 60°C·h⁻¹ (dotted line), 30°C·h⁻¹ (dashed line), and 15°C·h⁻¹ (solid line) in the 40–65° interval. Inset: detail of the thermogram at 60°C·h⁻¹ in the 31–79°C interval. (b) Variation of the scan rate (β) with the apparent . The line is a fit by linear regression. (c) Determination of the activation energy through the scan rate dependence on (solid line) by fitting data to (3).

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