Effect of Temperature on Xylanase II from Trichoderma reesei QM 9414: A Calorimetric, Catalytic, and Conformational Study

Figure 5

Reversible thermal unfolding of xylanase followed by DSC. (a) Variation of the excess heat capacity of 0.56 mg·mL⁻¹ xylanase with temperature at 60°C·h⁻¹ scan rate. The data (black dotted line) were fitted to the non-two-state model obtaining the cumulative Gauss curve through the data (red solid line). Six blue solid lines were obtained by deconvolution analysis with (4). (b) Same as in (a) except that the data correspond to the second scan of the sample. (c) Dependence of on xylanase concentration, 0.4 and 0.56 mg·mL⁻¹ (reversible process), and of apparent on 0.8 and 1 mg·mL⁻¹ (irreversible process). The line is an itered fit of the data to (5).

(a)

(b)

(c)