Research Article
Optimization of Enzymatic Saccharification of Alkali Pretreated Parthenium sp. Using Response Surface Methodology
Table 3
Experimental design and results of CCD for enzymatic hydrolysis of pretreated Parthenium sp.
| Run number | : temperature (°C) | : pH | : enzyme loading (mL) | : substrate loading (g) | Saccharification efficiency (%) | Experimental | Predicted |
| 1 | 50 | 4.25 | 0.8 | 0.2 | 72.52 | 75.16 | 2 | 60 | 4.25 | 0.4 | 0.4 | 17.38 | 20.40 | 3 | 50 | 4.75 | 0.4 | 0.4 | 46.88 | 39.52 | 4 | 55 | 4.5 | 0.6 | 0.3 | 54.73 | 54.73 | 5 | 60 | 4.25 | 0.8 | 0.2 | 25.97 | 29.81 | 6 | 50 | 4.25 | 0.4 | 0.2 | 61.51 | 57.78 | 7 | 55 | 4.5 | 0.6 | 0.1 | 35.67 | 36.52 | 8 | 60 | 4.25 | 0.8 | 0.4 | 23.21 | 24.78 | 9 | 55 | 4.5 | 1.0 | 0.3 | 71.52 | 64.83 | 10 | 50 | 4.25 | 0.8 | 0.4 | 59.14 | 56.79 | 11 | 45 | 4.5 | 0.6 | 0.3 | 68.50 | 75.31 | 12 | 60 | 4.75 | 0.8 | 0.2 | 22.16 | 28.07 | 13 | 55 | 4.5 | 0.2 | 0.3 | 28.41 | 37.07 | 14 | 55 | 4.5 | 0.6 | 0.3 | 54.73 | 54.73 | 15 | 50 | 4.75 | 0.8 | 0.2 | 83.27 | 76.73 | 16 | 60 | 4.75 | 0.8 | 0.4 | 23.67 | 23.87 | 17 | 60 | 4.75 | 0.4 | 0.2 | 15.15 | 13.98 | 18 | 60 | 4.25 | 0.4 | 0.2 | 26.29 | 21.71 | 19 | 65 | 4.5 | 0.6 | 0.3 | 8.78 | 3.94 | 20 | 55 | 4.5 | 0.6 | 0.5 | 16.52 | 17.66 | 21 | 55 | 5.0 | 0.6 | 0.3 | 38.12 | 39.10 | 22 | 55 | 4.0 | 0.6 | 0.3 | 43.45 | 44.44 | 23 | 55 | 4.5 | 0.6 | 0.3 | 54.73 | 54.73 | 24 | 50 | 4.75 | 0.4 | 0.2 | 53.37 | 53.35 | 25 | 55 | 4.5 | 0.6 | 0.3 | 54.73 | 54.73 | 26 | 55 | 4.5 | 0.6 | 0.3 | 54.73 | 54.73 | 27 | 50 | 4.75 | 0.8 | 0.4 | 53.05 | 59.18 | 28 | 50 | 4.25 | 0.4 | 0.4 | 47.47 | 43.12 | 29 | 60 | 4.75 | 0.4 | 0.4 | 14.59 | 13.50 | 30 | 55 | 4.5 | 0.6 | 0.3 | 54.73 | 54.73 |
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