Optimization of Enzymatic Saccharification of Alkali Pretreated Parthenium sp. Using Response Surface Methodology

Table 4

ANOVA for quadratic response surface model (RSM) from enzymatic saccharification of pretreated Parthenium sp.

Source	Sum of squares	Degree of freedom	Mean square	𝐹 value	P value (Prob > 𝐹)
Model	11340.56	14	810.04	26.51	<0.0001
𝐴: temperature	7640.87	1	7640.87	250.04	<0.0001
𝐵: pH	42.69	1	42.69	1.40	0.2556
𝐶: enzyme loading	1155.07	1	1155.07	37.83	<0.0001
𝐷: substrate loading	533.46	1	533.46	17.45	0.0008
𝐴𝐵	10.91	1	10.91	0.36	0.5591
𝐴𝐶	86.26	1	86.26	2.82	0.1136
𝐴𝐷	178.29	1	178.29	5.83	0.0289
𝐵𝐶	35.91	1	35.91	1.18	0.2955
𝐵𝐷	0.69	1	0.69	0.023	0.8823
𝐶𝐷	13.78	1	13.78	0.45	0.5121
𝐴^2	391.03	1	391.03	12.80	0.0028
𝐵^2	287.84	1	287.84	9.42	0.0078
𝐶^2	24.47	1	24.47	0.80	0.3850
𝐷^2	1310.41	1	1310.41	42.88	<0.0001
Residual	458.38	15	30.56
Lack of fit	458.38	10	45.84
Pure error	0.00	5	0.00
Total	11798.95	29
SD	5.53		𝑅^2	0.96
Mean	42.83		Adjusted 𝑅^2	0.93
CV(%)	12.91		Predicted 𝑅^2	0.78