Immobilization of Papain on Chitin and Chitosan and Recycling of Soluble Enzyme for Deflocculation of Saccharomyces cerevisiae from Bioethanol Distilleries
Table 6
Total activity of papain in several steps, according to each protocol of immobilization.
Immobilization protocola
Total enzyme in solution
Enzyme solution after the immobilization process
1st pellets washing-water (100 mL)
2nd pellets washing-water (1000 mL)
Active immob. enzyme (U)c
A
355.14
215.88 (14)b
74.0
0.0
1.53 (6.5)d
B
481.34
333.48 (14)
136.0
0.0
0.72 (6.5)
C
416.86
122.46 (13)
148.0
66.7
0.43 (6.5)
D
316.00
132.66 (18)
48.0
0.0
0.00 (7.5)
E
360.94
240.79 (18)
70.3
31.0
0.00 (7.5)
F
424.46
225.54 (18)
87.0
0.0
0.10 (7.5)
A′-5 h
206.50
55.97 (39)
0.0
0.0
5.76 (2.78)
B′-5 h
180.65
155.61 (26)
0.0
0.0
3.94 (3.17)
C′-5 h
201.00
138.13 (33)
0.0
0.0
2.24 (2.70)
A′-12 h
184.75
34.59 (30)
0.0
0.0
2.21 (2.66)
B′-12 h
197.50
6.65 (25)
0.0
0.0
3.72 (2.86)
C′-12 h
197.75
29.92 (34)
0.0
0.0
2.34 (2.84)
aProtocols in Tables 1 and 2; bremaining volume (mL) obtained from each immobilization is indicated in parentheses; ctotal units of immobilized enzyme, and dwet weight (g) of pellets.
