Research Article
An Efficient and Improved Methodology for the Screening of Industrially Valuable Xylano-Pectino-Cellulolytic Microbes
Table 1
Zones of substrate hydrolysis and activity analysis of xylano-pectino-cellulolytic enzymes.
| | Isolates | Qualitative analysis | Quantitative analysis |
Diameter (mm) of zone of substrate
hydrolysis on agricultural wastes |
Diameter (mm) of zone of substrate
hydrolysis on commercial substrates |
Enzyme activity
nkat/mLa | | Wheat bran | Citrus peel | Waste paper | Xylan | Pectin | Cellulose | Xylanase | Pectinase | Cellulase |
| | AVS 1 | 27 ± 1 | 20 ± 1 | 5 ± 1 | 28 ± 1 | 21 ± 1 | 5 ± 1 | 167 ± 28 | 134 ± 17 | 3.5 ± 0.5 | | AVS 2 | 22 ± 1 | 20 ± 2 | 13 ± 1 | 22 ± 1 | 20 ± 1 | 14 ± 3 | 83 ± 16 | 117 ± 13 | 22 ± 1.5 | | AVS 3 | 31 ± 1 | 14 ± 1 | 5 ± 1 | 32 ± 1 | 15 ± 1 | 5 ± 1 | 233 ± 35 | 50 ± 8 | 3.2 ± 0.6 | | AVS 4 | 20 ± 2 | 23 ± 2 | 18 ± 1 | 20 ± 1 | 24 ± 2 | 18 ± 1 | 69 ± 8 | 184 ± 26 | 30 ± 3.5 | | AVS 5 | 25 ± 1 | 19 ± 1 | 25 ± 1 | 26 ± 1 | 19 ± 1 | 26 ± 1 | 150 ± 18 | 101 ± 18 | 42 ± 4 | | AVS 6 | 24 ± 1 | 14 ± 1 | 29 ± 2 | 24 ± 1 | 14 ± 1 | 30 ± 3 | 117 ± 14 | 50 ± 8 | 54 ± 5.2 | | AVS 7 | 32 ± 1 | 13 ± 1 | 26 ± 1 | 33 ± 1 | 13 ± 1 | 26 ± 1 | 267 ± 30 | 25 ± 5 | 41 ± 5 | | AVS 8 | 38 ± 1 | 11 ± 1 | 6 ± 1 | 39 ± 2 | 11 ± 1 | 6 ± 1 | 852 ± 62 | 20 ± 3.5 | 5 ± 0.8 | | AVS 9 | 28 ± 1 | 14 ± 1 | 10 ± 1 | 28 ± 1 | 13 ± 1 | 10 ± 1 | 184 ± 31 | 35 ± 4 | 17 ± 1 | | AVS 10 | 18 ± 2 | 16 ± 1 | 7 ± 1 | 17 ± 1 | 16 ± 1 | 7 ± 1 | 35 ± 7 | 67 ± 9 | 8.5 ± 0.8 | | AVS 11 | 17 ± 1 | 29 ± 3 | 12 ± 1 | 17 ± 1 | 30 ± 3 | 12 ± 1 | 25 ± 3.5 | 434 ± 41 | 20 ± 1.5 | | AVS 12 | 14 ± 1 | 11 ± 1 | 8 ± 1 | 14 ± 1 | 10 ± 1 | 8 ± 1 | 9 ± 1.5 | 17 ± 2.5 | 11 ± 1 | | AVS 13 | 34 ± 1 | 26 ± 2 | 32 ± 2 | 35 ± 1 | 26 ± 2 | 32 ± 1 | 368 ± 36 | 301 ± 22 | 100 ± 16 | | AVS 14 | 19 ± 2 | 23 ± 1 | 22 ± 1 | 19 ± 1 | 24 ± 1 | 24 ± 1 | 57 ± 11 | 167 ± 18 | 40 ± 4 | | AVS 15 | 32 ± 1 | 18 ± 1 | 11 ± 1 | 34 ± 1 | 18 ± 1 | 10 ± 1 | 284 ± 17 | 84 ± 9 | 14 ± 1.2 | | AVS 16 | 32 ± 1 | 13 ± 1 | 7 ± 1 | 33 ± 2 | 13 ± 1 | 7 ± 1 | 267 ± 29 | 34 ± 3.5 | 8 ± 1 | | AVS 17 | 32 ± 1 | 20 ± 2 | 22 ± 1 | 33 ± 1 | 21 ± 2 | 22 ± 3 | 251 ± 14 | 117 ± 14 | 30 ± 3 | | AVS 18 | 20 ± 3 | 8 ± 1 | 26 ± 1 | 20 ± 1 | 9 ± 1 | 27 ± 1 | 69 ± 10 | 9 ± 1 | 45 ± 4.2 | | AVS 19 | 24 ± 1 | 20 ± 1 | 5 ± 1 | 24 ± 1 | 21 ± 1 | 6 ± 1 | 117 ± 16 | 104 ± 15 | 3.5 ± 0.5 | | AVS 20 | 30 ± 1 | 8 ± 1 | 18 ± 1 | 31 ± 1 | 9 ± 1 | 18 ± 1 | 217 ± 25 | 14 ± 1.5 | 25 ± 2.5 | | AVS 21 | 30 ± 1 | 13 ± 1 | 12 ± 1 | 30 ± 1 | 14 ± 1 | 12 ± 1 | 201 ± 17 | 50 ± 6 | 20 ± 1.5 | | AVS 22 | 34 ± 1 | 16 ± 1 | 17 ± 1 | 35 ± 1 | 16 ± 1 | 17 ± 1 | 384 ± 33 | 58 ± 10 | 27 ± 2 | | AVS 23 | 34 ± 2 | 13 ± 1 | 26 ± 1 | 35 ± 3 | 13 ± 1 | 27 ± 1 | 401 ± 28 | 34 ± 3.5 | 35 ± 3.5 | | AVS 24 | 22 ± 1 | 10 ± 1 | 14 ± 1 | 23 ± 1 | 11 ± 1 | 14 ± 1 | 84 ± 0.9 | 13 ± 1.5 | 24 ± 2.5 | | AVS 25 | 31 ± 1 | 12 ± 1 | 8 ± 1 | 32 ± 1 | 12 ± 1 | 8 ± 1 | 234 ± 20 | 20 ± 2 | 12 ± 1.2 | | AVS 26 | 30 ± 2 | 15 ± 1 | 10 ± 1 | 31 ± 1 | 16 ± 1 | 10 ± 1 | 217 ± 18 | 42 ± 5 | 14 ± 1.5 | | AVS 27 | 19 ± 1 | 13 ± 1 | 5 ± 1 | 20 ± 1 | 13 ± 1 | 6 ± 1 | 58 ± 6 | 27 ± 3 | 3.2 ± 0.8 | | AVS 28 | 34 ± 1 | 26 ± 3 | 27 ± 1 | 35 ± 2 | 28 ± 2 | 28 ± 1 | 317 ± 32 | 267 ± 31 | 44 ± 4.5 | | AVS 29 | 31 ± 1 | 24 ± 1 | 33 ± 2 | 32 ± 1 | 25 ± 2 | 34 ± 1 | 234 ± 21 | 217 ± 26 | 92 ± 13 | | AVS 30 | 30 ± 1 | 9 ± 1 | 29 ± 3 | 30 ± 1 | 8 ± 1 | 30 ± 2 | 201 ± 13 | 9 ± 0.8 | 67 ± 8.5 | | AVS 31 | 32 ± 1 | 24 ± 2 | 30 ± 1 | 33 ± 2 | 25 ± 1 | 30 ± 1 | 267 ± 27 | 200 ± 17 | 54 ± 6 | | AVS 32 | 25 ± 1 | 18 ± 1 | 7 ± 1 | 25 ± 1 | 18 ± 1 | 8 ± 1 | 134 ± 12 | 87 ± 8 | 12 ± 1 | | AVS 33 | 32 ± 2 | 15 ± 1 | 23 ± 1 | 32 ± 1 | 15 ± 1 | 24 ± 1 | 251 ± 15 | 39 ± 4 | 35 ± 3.2 | | AVS 34 | 35 ± 2 | 25 ± 1 | 5 ± 1 | 37 ± 1 | 25 ± 1 | 5 ± 1 | 585 ± 61 | 184 ± 17 | 3.5 ± 0.8 | | AVS 35 | 30 ± 1 | 24 ± 2 | 27 ± 1 | 31 ± 1 | 24 ± 3 | 28 ± 1 | 217 ± 30 | 201 ± 25 | 44 ± 4 | | AVS 36 | 17 ± 2 | 8 ± 1 | 13 ± 1 | 18 ± 1 | 8 ± 1 | 14 ± 1 | 27 ± 3.2 | 12 ± 1 | 21 ± 2 | | AVS 37 | 32 ± 1 | 18 ± 1 | 22 ± 2 | 32 ± 1 | 19 ± 1 | 23 ± 1 | 234 ± 23 | 100 ± 17 | 34 ± 3.5 | | AVS 38 | 20 ± 1 | 16 ± 1 | 15 ± 1 | 20 ± 1 | 16 ± 1 | 16 ± 1 | 67 ± 14 | 54 ± 8 | 25 ± 2 | | AVS 39 | 32 ± 1 | 14 ± 1 | 8 ± 1 | 32 ± 2 | 15 ± 1 | 8 ± 1 | 251 ± 25 | 35 ± 3.5 | 12 ± 1.2 | | AVS 40 | 32 ± 1 | 15 ± 2 | 22 ± 1 | 32 ± 1 | 15 ± 2 | 24 ± 1 | 234 ± 20 | 51 ± 5 | 36 ± 4 | | AVS 41 | 34 ± 1 | 8 ± 1 | 29 ± 2 | 35 ± 1 | 8 ± 1 | 30 ± 3 | 351 ± 33 | 8 ± 0.75 | 54 ± 5 | | AVS 42 | 36 ± 3 | 14 ± 1 | 6 ± 1 | 38 ± 2 | 14 ± 1 | 6 ± 1 | 702 ± 42 | 40 ± 3.5 | 7 ± 1.5 | | AVS 43 | 20 ± 1 | 24 ± 1 | 6 ± 1 | 20 ± 1 | 25 ± 1 | 6 ± 1 | 67 ± 16 | 201 ± 23 | 5 ± 1 | | AVS 44 | 25 ± 2 | 28 ± 2 | 8 ± 1 | 25 ± 1 | 30 ± 2 | 8 ± 1 | 134 ± 21 | 434 ± 37 | 10 ± 1.5 | | AVS 45 | 19 ± 1 | 26 ± 1 | 7 ± 1 | 19 ± 1 | 27 ± 3 | 7 ± 1 | 50 ± 7 | 267 ± 28 | 9 ± 1.2 | | AVS 46 | 32 ± 2 | 26 ± 1 | 33 ± 2 | 33 ± 1 | 27 ± 1 | 34 ± 2 | 267 ± 24 | 117 ± 16 | 82 ± 15 | | AVS 47 | 35 ± 2 | 14 ± 1 | 8 ± 1 | 36 ± 1 | 14 ± 1 | 8 ± 1 | 518 ± 38 | 34 ± 3.5 | 10 ± 1.5 | | AVS 48 | 32 ± 1 | 15 ± 1 | 13 ± 1 | 32 ± 1 | 16 ± 1 | 13 ± 1 | 251 ± 23 | 57 ± 5 | 21 ± 2 | | AVS 49 | 25 ± 1 | 14 ± 1 | 8 ± 1 | 25 ± 1 | 15 ± 1 | 8 ± 1 | 134 ± 16 | 50 ± 4 | 8.5 ± 1.2 | | AVS 50 | 30 ± 1 | 8 ± 1 | 22 ± 1 | 31 ± 1 | 8 ± 1 | 23 ± 2 | 217 ± 20 | 14 ± 1.5 | 37 ± 3.2 |
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Under unoptimized conditions.
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