Research Article

Chloride Activated Halophilic α-Amylase from Marinobacter sp. EMB8: Production Optimization and Nanoimmobilization for Efficient Starch Hydrolysis

Figure 2

Effect of nitrogen sources on growth and amylase production. Medium (g/L: starch, 50.0; K2HPO4, 0.87; MgSO4·7H2O, 6.2; KCl, 0.75; NaCl, 50.0; pH 7.0) was supplemented with different nitrogen sources at concentration so as to provide 1% (w/v) nitrogen. Medium was inoculated with 1% mother culture and incubation was carried out at 35°C for 72 h at 200 rpm shaking.