Plackett-Burman Design for rGILCC1 Laccase Activity Enhancement in Pichia pastoris: Concentrated Enzyme Kinetic Characterization

Figure 2

Treatment PBED kinetic follow-up. Treatment 1 [PBED-: 500 mL Erlenmeyer flask containing 150 mL media, 10% inoculum (v/v), 1.0 mM CuSO₄, 30 gL⁻¹ glucose, 5 mM NH₄SO₄, 10 gL⁻¹ peptone, and 5 gL⁻¹ yeast extract], enzy. act. 4.69 ± 0.39 UL⁻¹ at 168 h of culture. Assay was carried out in triplicate ().