Plackett-Burman Design for rGILCC1 Laccase Activity Enhancement in Pichia pastoris: Concentrated Enzyme Kinetic Characterization

Figure 3

rGILCC1 concentrate characterization. (a) Zymogram gel for rGILCC1 enzyme functional identification. As positive control, Laccase Lac (Sigma-Aldrich). (b) Relative enzyme activity (%) as a function of rGILCC1 obtained from concentrate after 1 h incubation at different temperatures and pH (assay carried out in triplicate, n = 3). Activity was determined as described in Materials and Methods [13]. (c) rGILCC1 obtained from concentrate enzyme kinetics using ABTS as a substrate (assay carried out in triplicate, n = 3). (d) (Michaelis-Menten constant), (maximal velocity) detailed values.

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