Plackett-Burman Design for rGILCC1 Laccase Activity Enhancement in Pichia pastoris: Concentrated Enzyme Kinetic Characterization

Table 1

Laccase activity ANOVA for a model that was not adjusted for curvature.

Source	Sum of squares	DF	Mean squares	F-value	p value
					Prob > F
Model	19.57	10	1.96	16.17	0.0034
A, culture media volume	1.17	1	1.17	9.71	0.0264
B, CuSO4	1.90	1	1.90	15.70	0.0107
D, glucose	0.089	1	0.089	0.74	0.4304
E, NH4SO4	0.07	1	0.07	0.58	0.4800
F, peptone	1.17	1	1.17	9.64	0.0267
G, yeast extract	1.60	1	1.60	13.24	0.0149
Residual	0.61	5	0.12
Lack of fit	0.05	2	0.025	0.14	0.8777
Error	0.55	3	0.18
Cor total	20.18	15
R-square	0.97
Adjusted R-square	0.91
Predicted R-square	0.7479
Adequate precision	16.258

95% significant values are in bold.