Plackett-Burman Design for rGILCC1 Laccase Activity Enhancement in Pichia pastoris: Concentrated Enzyme Kinetic Characterization

Table 3

PBED evaluated factor effect and percentage contribution on laccase activity.

Factor	Effect	Sum of squares	p	%; contribution
, culture media volume	−0.63	1.17	0.0264	5.81
, CuSO4	0.80	1.90	0.0107	9.39
, glucose	0.17	0.09	0.4304	0.44
, NH4SO4	0.15	0.07	0.4800	0.35
, peptone	0.62	1.17	0.0267	5.76
, yeast extract	−0.73	1.60	0.0149	7.92

Significant values are in bold.