Comparative Study of Cyanobacterial and E. coli RNA Polymerases: Misincorporation, Abortive Transcription, and Dependence on Divalent Cations
Figure 1
Cyanobacterial RNAPs. (a) Analysis of the fractions of The core enzyme by 10% SDS-PAGE after the treatment of hydrophobic resin (lane 1 : 26.9 μg), DEAE anion-exchange (lane 2 : 7.0 μg), heparin affinity (lane 3 : 0.8 μg), and MonoQ chromatography (lane 4 : 1.6 μg). Lanes 5–7 show 7.5 μg of the purified The, Syn, and E. coli enzymes used in transcription assays. Marker proteins (BIO-RAD) are also shown in lane M with the molecular weights indicated in the left margin. The gel was stained with Coomasie Brriliant Blue. (b) Schematic diagrams of β′ subunits of The, Syn, a chloroplast of red alga Porphyra purpurea, and E. coli. The conserved regions A–H [11, 12] were also indicated. The white boxes represent the nonconserved domain inserted in Region G [13, 14]. The split sites are indicated by an arrow. The scale bar of 100 amino-acid residues (aa) is indicated at the bottom.
