Comparative Study of Cyanobacterial and E. coli RNA Polymerases: Misincorporation, Abortive Transcription, and Dependence on Divalent Cations
Figure 3
Inverse pulse-labeling assay for detecting persistent abortive synthesis at the psbA2 promoter by Syn RNAP. (a) Schematic diagram of the assay. The reaction time is varied. (b) The labeled products synthesized at 1 mM Mn2+ (left) and at 1 mM Mg2+ (right). The runoff transcript and the 6-mer abortive transcript are indicated by arrowheads. (c) The ratios of the amount of 6-mer abortive transcript to that of the runoff transcript were plotted against the reaction time .
