Ontogenetic Survey of Histone Modifications in an Annelid

Figure 3

H3K14 acetylation in larvae and adults of Polydora cornuta. (a–c) Companion micrographs of the ventral surface of a three-chaetiger larva (A-brood) in bright field (a), showing nuclear DNA (b, DAPI), and showing nuclei that are acetylated at H3K14 (c, FITC-conjugated anti-H3K14ac). Note the strong epifluorescence that is typical of cells of the mouth and pygidium (small arrows in c). (d–f) Dorsal view of a four-chaetiger larva (A-brood) in bright field (d), with DAPI (e) and with FITC-conjugated anti-H3K14ac (f). (g, h) Images of an ectodermal cell from a gastrula (P-brood) that is undergoing mitosis, double labeled with DAPI (g) and FITC-conjugated anti-H3K14ac (h). (i, j) Paraffin section through the body wall of a female that is double labeled with DAPI (i) and FITC-conjugated anti-H3K14ac (j). cs: chaetal sac, ch: chaetae, e: eye, ep: epidermis, g: gut, h: head, ls: larval spines, m: mouth, n: nephridium, s: segment, and p: pygidium. Arrows indicate the presence of FITC-conjugated H3K14ac in the indicated cells. Scale bars = 10 μm (g, h) or 50 μm (a–f, i, j).