Lack of TEK Gene Mutation in Patients with Cutaneomucosal Venous Malformations from the North-Western Region of Algeria

Table 1

Primer sequences of all the exons of the TEK gene.

Exon	Amorce sens (5′-3′)	Amorce antisens (5′-3′)	PCR product
Exon1.1	AGTCTGAGAAGGATTGGTCATCA	CTGTCTGAGCACAGGGAGTTT	333 bp
Exon1.2	CAGCCCTGCTGATACCAAAT	CACTGATGAGATTTGGGGAGA	409 bp
Exon2	GTTTACCCAATGGGGTCATG	AGCAGCTGCCAAGACAAAAG	448 bp
Exon3	AACGCATTAGCCACCACTGT	ACATCTGCCCACAAGACCA	360 bp
Exon4	CTGAATAGTTCAGCATTTTCATTCT	CAATGCCTGGTTTTTGCTTA	422 bp
Exon5	CTCCTTGTCTTTGTTTCTGTCG	AAATTCTAGATCCAGCAACGATG	399 bp
Exon6	GTTCATCCTACCATGCCACA	TGATTCAAAATCCTGTTGTCCA	413 bp
Exon7	AGTTGGCATGATAGGAGCTCA	GGATGGAAACAAAAGAGGCTT	453 bp
Exon8	TCATCCACATCACAGGTGTCT	GTCAGTTCTGCCTCTCCAGG	469 bp
Exon9	TGGGGTCAATGTTATGGACC	TCCTGGAAATTACCCCAAAG	335 bp
Exon10	ATCACAAAACCTCAAAGCCG	AGCCACCACCTTGAGGTAGA	331 bp
Exon11	TTTCAAAAGCCTAATTTTCCTCA	CACCCATTCAAAAGCGAACT	462 bp
Exon12.1	AGTTGGCATGATAGGAGCTCA	GGATGGAAACAAAAGAGGCTT	453 bp
Exon12.2	TGGGGTCAATGTTATGGACC	TCCTGGAAATTACCCCAAAG	335 bp
Exon13	GCATAATGATCTAGGCCATGG	CCTATAGGGCTGCACGGTAA	413 bp
Exon14	GCTGCTGTTAAGTTCCCATTACA	AAGCCAAAGAGAAGATGAGGC	397 bp
Exon15	GTTCATCCTACCATGCCACA	TGATTCAAAATCCTGTTGTCCA	413 bp
Exon16	TTTGGTTGTATACAGTTGATGGTGA	AGGCAAACCACAGCACAGTC	404 bp
Exon17	GTTTACCCAATGGGGTCATG	AGCAGCTGCCAAGACAAAAG	448 bp
Exon18	TCTTCTGCCAAGATGTGGTG	CAGGGGAGTACCTCGGAAA	358 bp
Exon19	CTACCCAGCAATCATTTGTGG	TGCTAATTTATTTCCTGAGCTTTTT	310 bp
Exon20	GTGCAAGGGCCTATCCTAGG	CCAAGTCACATCTGGTAGAACCA	304 bp
Exon21	ATGTGCAGTGAGTTTGCCAA	CGGCTGACTTTGCTAGAGTC	338 bp
Exon22	GTTTACCCAATGGGGTCATG	AGCAGCTGCCAAGACAAAAG	448 bp
Exon23.1	AGGTGGAATCAAAGCAGCCT	CACGCCTTCCTATGAAGTCC	414 bp
Exon23.2	AATCAGAATGCCTGTTTGTGG	TTCTTAGGCTTGTAAGCAATGAG	452 bp
3′UTR region	TCTCAATTTTATCCCTCACCTG	TAAAGTATAATAAGGACATGTGGCA	472 bp

bp: base pair.
3′UTR: 3′ untranslated region.