Genetics Research International

Research Article

Molecular Genetic Diversity and Quantitation of Methanogen in Ruminal Fluid of Buffalo (Bubalus bubalis) Fed Ration (Wheat Straw and Concentrate Mixture Diet)

Table 1

Similarity values of 16S rRNA gene sequences retrieved from rumen of Surti buffalo.
Phylotypes (OTUs)Clone no.Accession no.GeneraNearest Taxa 
(Accession no.)		Identity
(%)
ORFSBRM1 HM566228Methanobrevibacter sp.Uncultured Methanobrevibacter sp.
(HM125682)
ORFSBRM2 HM566229Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616023.1
ORFSBRM3 HM566230Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616006.1
ORFSBRM4 HM566231N/AUncultured archaeon clone sy-904231054-77-i
GQ255495.1
ORFSBRM5 HM566232Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616023.1
ORFSBRM6 HM566233N/AUncultured archaeon clone sy-904231051-84-i
GQ255499.1
ORFSBRM7 HM566234Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616006.1
ORFSBRM8 HM566235N/AUncultured methanogenic archaeon
EU794774.1
ORFSBRM9 HM566236N/AUncultured archaeon clone sy-904231051-40-i
GQ255534.1
ORFSBRM10 HM566237N/AUncultured archaeon clone sy-904231051-84-i
GQ255499.1
ORFSBRM11 HM566238Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616023.1
ORFSBRM12 HM566239Methanomicrobium sp.Uncultured Methanomicrobium sp.
HM038371.1
ORFSBRM13 HM566240Methanobrevibacter sp.Uncultured Methanobrevibacter sp.
FJ919272.1
ORFSBRM14 HM566241Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616023.1
ORFSBRM15 HM566242Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616006.1
ORFSBRM16 HM566243Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616023.1
ORFSBRM17 HM566244Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616023.1
ORFSBRM18 HM566245Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616023.1
ORFSBRM19 HM566246Methanomicrobium sp.Uncultured Methanomicrobium sp.
HQ616023.1
ORFSBRM20 HM566247Methanomicrobium mobile Methanomicrobium mobile  
M59142.1
ORFSBRM21 HM566248Uncultured Methanobacteriales Uncultured Methanobacteriales archaeon DQ402018.1
Total clones = 76.