Research Article
Frequency Distribution of Mannose Binding Lectin-2 and Vitamin D Receptor Gene Variants: Putative Markers for Tuberculosis
Table 2
The restriction digestion reaction conditions and genotype assignment after the digestion.
| SNP | Incubation Temperature | Homozygous wild type | Heterozygote | Homozygous mutant | % agarose gel |
| FokI | 37°C | 265 bp | 265 bp, 195 bp, 70 bp | 195 bp, 70 bp | 2% | BsmI | 37°C | 160 bp, 235 bp | 388 bp, 160 bp, 235 bp | 388 bp | 2% | TaqI | 65°C | 485 bp | 485 bp, 297 bp, 188 bp | 297 bp, 188 bp | 2% | ApaI | 37°C | 1174 bp, 217 bp | 1391 bp, 1174 bp, 217 bp | 1391 bp | 1.5% |
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