Caspase Activation and Aberrant Cell Growth in a p53^+/+ Cell Line from a Li-Fraumeni Syndrome Family

Figure 2

Caspase activity assay after 120 minutes of reaction. Apoptosis was induced by adding 150 μM (final concentration) etoposide into culture medium for various hours as indicated. Medium was replaced with fresh etoposide every 24 hours. Cells were collected by trypsinization. Apoptosis was analyzed using Caspase-3 Fluorometric Assay kit (Cat. number K105-100) from Biovision (Mountain View, CA) according to the manufacturer’s instructions. Results were analyzed by fluorometer. Data were shown as relative fluorescence units (shown on the -axis). A representative experiment of three independent replicates is shown.