Leveraging Comparative Genomics to Identify and Functionally Characterize Genes Associated with Sperm Phenotypes in Python bivittatus (Burmese Python)
Table 2
Gene ontology enrichment.
Category
GO identifier
Annotation term
Count
Enrichment
value
Biological process
GO:0019953
Sexual reproduction
45
13.99
7.20E − 40
Biological process
GO:0007276
Gamete generation
41
14.78
7.70E − 37
Biological process
GO:0048232
Male gamete generation
37
17.11
3.46E − 35
Biological process
GO:0007283
Spermatogenesis
37
17.11
3.46E − 35
Biological process
GO:0032504
Multicellular organism reproduction
42
12.28
1.54E − 34
Biological process
GO:0048609
Reproductive process in a multicellular organism
42
12.28
1.54E − 34
Biological process
GO:0003006
Reproductive developmental process
27
14.67
2.70E − 23
Biological process
GO:0048610
Reproductive cellular process
22
19.34
2.55E − 21
Biological process
GO:0007281
Germ cell development
18
25.38
2.20E − 19
Biological process
GO:0048515
Spermatid differentiation
14
34.98
6.97E − 17
Biological process
GO:0007286
Spermatid development
13
34.28
1.62E − 15
Biological process
GO:0007548
Sex differentiation
15
14.15
2.05E − 12
Biological process
GO:0045137
Development of primary sexual characteristics
12
13.46
1.15E − 09
Biological process
GO:0008406
Gonad development
11
13.99
5.09E − 09
Biological process
GO:0048608
Reproductive structure development
11
12.43
1.60E − 08
Biological process
GO:0046661
Male sex differentiation
9
17.56
3.73E − 08
Biological process
GO:0051327
M phase of meiotic cell cycle
9
13.08
3.79E − 07
Biological process
GO:0007126
Meiosis
9
13.08
3.79E − 07
Biological process
GO:0051321
Meiotic cell cycle
9
12.82
4.43E − 07
Biological process
GO:0046546
Development of primary male sexual characteristics
7
15.34
5.69E − 06
Biological process
GO:0009566
Fertilization
7
12.62
1.77E − 05
Biological process
GO:0008584
Male gonad development
6
16.43
2.92E − 05
Cellular component
GO:0005929
Cilium
7
8.89
1.24E − 04
Cellular component
GO:0031514
Motile secondary cilium
3
122.90
2.13E − 04
Cellular component
GO:0042995
Cell projection
14
3.29
2.44E − 04
Cellular component
GO:0001669
Acrosomal vesicle
4
17.72
1.41E − 03
Cellular component
GO:0019861
Flagellum
4
14.25
2.65E − 03
Cellular component
GO:0030141
Secretory granule
6
5.46
4.57E − 03
Cellular component
GO:0016023
Cytoplasmic membrane-bounded vesicle
10
2.98
5.65E − 03
Cellular component
GO:0031988
Membrane-bounded vesicle
10
2.89
6.93E − 03
Cellular component
GO:0005625
Soluble fraction
7
3.66
1.14E − 02
Cellular component
GO:0031410
Cytoplasmic vesicle
10
2.55
1.47E − 02
Cellular component
GO:0015630
Microtubule cytoskeleton
9
2.69
1.72E − 02
Cellular component
GO:0031982
Vesicle
10
2.45
1.89E − 02
Cellular component
GO:0000267
Cell fraction
13
1.97
2.79E − 02
Cellular component
GO:0033391
Chromatoid body
2
54.62
3.56E − 02
Cellular component
GO:0060293
Germ plasm
2
46.82
4.14E − 02
Cellular component
GO:0045495
Pole plasm
2
46.82
4.14E − 02
Cellular component
GO:0043186
P granule
2
46.82
4.14E − 02
Cellular component
GO:0034464
BBSome
2
46.82
4.14E − 02
Cellular component
GO:0060170
Cilium membrane
2
40.97
4.72E − 02
Molecular function
GO:0046983
Protein dimerization activity
13
3.71
1.60E − 04
Molecular function
GO:0042802
Identical protein binding
14
3.38
1.96E − 04
Molecular function
GO:0043565
Sequence-specific DNA binding
12
3.06
1.61E − 03
Molecular function
GO:0003707
Steroid hormone receptor activity
4
12.62
3.76E − 03
Molecular function
GO:0015631
Tubulin binding
5
7.73
3.82E − 03
Molecular function
GO:0030554
Adenyl nucleotide binding
20
1.96
4.37E − 03
Molecular function
GO:0001883
Purine nucleoside binding
20
1.93
5.16E − 03
Molecular function
GO:0042803
Protein homodimerization activity
8
3.70
5.46E − 03
Molecular function
GO:0001882
Nucleoside binding
20
1.92
5.55E − 03
Molecular function
GO:0004879
Ligand-dependent nuclear receptor activity
4
10.66
6.04E − 03
Molecular function
GO:0030528
Transcription regulator activity
19
1.94
6.36E − 03
Molecular function
GO:0003700
Transcription factor activity
14
2.22
8.63E − 03
Molecular function
GO:0046982
Protein heterodimerization activity
6
4.46
1.06E − 02
Molecular function
GO:0005524
ATP binding
18
1.88
1.12E − 02
Molecular function
GO:0047485
Protein N-terminus binding
4
8.35
1.18E − 02
Molecular function
GO:0032559
Adenyl ribonucleotide binding
18
1.86
1.27E − 02
Molecular function
GO:0004672
Protein kinase activity
10
2.55
1.50E − 02
Molecular function
GO:0016563
Transcription activator activity
8
3.02
1.58E − 02
Molecular function
GO:0004674
Protein serine/threonine kinase activity
8
2.88
2.00E − 02
Gene Ontology Enrichment. The set of P. bivittatus identified as having sperm associated phenotypes were further explored to identify enrichment of gene ontology (GO) terms. A subset of the results are shown above, and the complete set of data is available in Supplemental File 3, Supplemental File 4, and Supplemental 5. Enrichment within the three categories of gene ontology (biological process, cell component, and molecular function) was identified. The displayed GO annotation terms correspond to the most significant values in each of the three categories. The number of genes associated with each annotation term is included, as are the value and the fold enrichment. The enrichment analysis was performed using the DAVID resource. Bonferroni corrected values and false discovery rate values are included in the tab-delimited supplemental files as well as the list of genes associated with each GO annotation term.
