Improved Recovery of Exfoliated Colonocytes from Feces Using Newly Developed Immunomagnetic Beads
Figure 2
A simulation experiment for the recovery rates of colonocytes depending on the affinity of mAbs. (a) The affinities of newly developed antihuman EpCAM mAbs are analyzed using flow cytometry. HT-29 cells, which are positive for EpCAM antigen, and UMUC-3 cells, which are negative for EpCAM antigen, are used. The white peak, black peak, dark-gray peak, and light-gray peak show nonspecific mouse IgG1 mAb, clone 1-2, clone B8-4, and clone B8-7, respectively. (b) Simulation examining the recovery of the colonocytes. The cell recovery rates using beads D (tagged with a lower affinity mAb, Clone 1-2), beads E (tagged with a higher affinity mAb, Clone B8-4), and beads F (tagged with a higher affinity mAb, Clone B8-7) are 4.5 ± 0.98 (%, mean ± SD), 73.5 ± 1.96, and 71.2 ± 3.39, respectively. The columns show the cell retrieval rates and the bars show the standard deviations. Significant differences are showed by an asterisk (*, P < .0001).
