| Metabolite | Adding amount (M) | Detection value (M) | Average recovery (%) | RSD (%) |
| Arginine | 20.0 | 16.0, 17.0, 18.0 | 85.00 | 5.88 | 50.0 | 43.0, 44.0, 46.0 | 88.70 | 3.45 | 80.0 | 68.0, 71.0, 73.0 | 83.33 | 2.29 | Histidine | 20.0 | 14.0, 15.0, 16.0 | 75.00 | 6.70 | 50.0 | 43.0, 44.0, 45.0 | 88.00 | 2.27 | 80.0 | 62.0, 65.0, 67.0 | 80.80 | 3.89 | Aspartate | 20.0 | 14.0, 15.0, 15.0 | 73.33 | 3.94 | 50.0 | 39.0, 41.0, 42.0 | 81.33 | 3.76 | 80.0 | 63.0, 65.0, 68.0 | 80.83 | 2.36 | Lactic acid | 20.0 | 15.5, 16.0, 17.3 | 81.33 | 5.71 | 50.0 | 42.6, 44.2, 43.0 | 86.53 | 1.92 | 80.0 | 68.5, 72.4, 70.2 | 87.96 | 2.78 | Citric acid | 20.0 | 13.6, 14.5, 15.0 | 71.83 | 4.94 | 50.0 | 39.4, 41.6, 43.0 | 82.67 | 4.39 | 80.0 | 68.3, 70.5, 74.0 | 88.67 | 4.05 |
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