Figure 1: RT-PCR analysis of FAF1 mRNA expression in gastric cancer tissue and matched normal gastric mucosa tissue. (a) RT-PCR to measure levels of FAF1 and -actin mRNA. Lanes 1, 3, 5: gastric cancer tissue from different patients. Lanes 2, 4, 6: corresponding normal gastric mucosa tissue from the same patients. M, 100-bp DNA marker ladder. β-actin was used as an internal control. (b) Comparison of FAF1 mRNA levels (normalized to β-actin levels) in gastric cancer tissue and matched normal gastric mucosa tissue. FAF1 mRNA levels were much lower in the gastric cancer tissue samples based on the mean SD test. .