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Gastroenterology Research and Practice
Volume 2012 (2012), Article ID 360929, 7 pages
http://dx.doi.org/10.1155/2012/360929
Research Article

Association between Genetic Instability and Helicobacter pylori Infection in Gastric Epithelial Dysplasia

1Department of Internal Medicine, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of Korea
2Department of Pathology, College of Medicine, The Catholic University of Korea, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of Korea
3The Research Institute of St. Vincent Hospital, 93 Jungbu-daero, Paldal-gu, Suwon 442-723, Republic of Korea
4Department of Pathology, Samsung Medical Center of Korea, 50 Ilwon-dong, Gangnam-gu, Seoul 135-710, Republic of Korea

Received 30 August 2012; Revised 27 November 2012; Accepted 3 December 2012

Academic Editor: Bita Geramizadeh

Copyright © 2012 Jin Su Kim et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Background. In gastric carcinogenesis, changes of DNA methylation appear to be an early molecular event, and the genome-wide methylation state is closely correlated with the level of long interspersed nucleotide element-1 (LINE-1) methylation. In this study, we measured LINE-1 methylation level according to genetic instability and evaluated the effect of Helicobacter pylori infection on genetic instability in gastric epithelial dysplasia. Methods. Total 100 tissue samples of gastric epithelial dysplasia were analyzed. Seven loci that linked to tumor suppressor genes were used to identify significant structural chromosomal aberrations. Microsatellite status was investigated for two different microsatellite marker loci (BAT25 and BAT26). Also, we measured LINE-1 methylation level by combined bisulfite restriction analysis (COBRA-LINE-1) method. Results. There were no significant differences of LINE-1 methylation level according to chromosomal/microsatellite instability and H. pylori state. In the dysplastic lesions with H. pylori infection, LINE-1 methylation level of MSI lesion was significantly lower than that of microsatellite stable (MSS) lesion ( versus %, ). Conclusions. In gastric epithelial dysplasia with H. pylori infection, MSI is correlated with reduced LINE-1 methylation level. Coexistence of H. pylori infection and MSI might be a driving force of gastric carcinogenesis.