Genetically Modified Lactococcus lactis for Delivery of Human Interleukin-10 to Dendritic Cells
Figure 2
(a) Immature DCs were not matured (grey histogram), matured with 1 × 105 cfu viable L. lactisIL-10 (continuous line), or matured with MF and 1 × 105 cfu viable L. lactisIL-10 (dotted line). CD83 (left panel) and CD86 (right panel) expression was assessed after 48 hours by flow cytometry. (b) Immature DCs were primed with MF or 1 × 105 cfu viable L. lactisIL-10 with and without MF. Depicted is the cell cycle progression of allogenic Th-cells co-cultured with effector Th-cells derived from MF-matured DC is regarded as 100% (white histograms, reference condition) and the cell cycle progression of allogenic Th-cells cocultured with effector Th-cells derived from L. lactisIL-10 (left panel) or L. lactisIL-10 /MF-(right panel) matured DC (gray histograms).
