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Figure 3: Autophagy inhibition reduced sulindac sulfide-induced apoptosis over time in serum-free media and increased sulfide-induced apoptosis over time in serum-containing media. HT-29 cells that were untransfected and transfected with control RNA and Atg7 siRNA were plated and treated as described in Figure 1. 3-MA was added to cell cultures 30 minutes prior to addition of sulindac sulfide. Apoptosis was determined by measuring fold caspase-3 activity. *Indicates significant reduction in fold caspase-3 activity in cell cultures treated with autophagy inhibitors and sulindac sulfide relative to sulindac sulfide only, . **Indicates significant increase in fold caspase-3 activity in cell cultures treated with autophagy inhibitors and sulindac sulfide relative to sulindac sulfide only, .