Regulation Effect of Zinc Fingers and Homeoboxes 2 on Alpha-Fetoprotein in Human Hepatocellular Carcinoma

Table 1

Primer sequences for RT-PCR analysis.

Gene	Primer sequences (5′-3′)	Annealing temperature (°C)	Cycle numbers	Amplified products (bp)
ZHX2	Sense: GGTAGCGACGAGAACGAG; Antisense: AGGACTTTGGCACTATGAAC	58	34	389
NF-YA	Sense: GAGTCTCGGCACCGTCAT; Antisense: TGCTTCTTCATCGGCTTG	57	34	117
AFP	Sense: GTTGCCAACTCAGTGAGGAC; Antisense: GAGCTTGGCACAGATCCTTA	59	28	240
GAPDH	Sense: GCTGAGAACGGGAAGCTTGT; Antisense: GCCAGGGGTGCTAAGCAGTT	58	30	299

AFP: alpha-fetoprotein, bp: base pair, GAPDH: glyceraldehyde-3-phosphate dehydrogenase, NF-Y: nuclear factor-Y, and ZHX2: zinc fingers and homeoboxes 2.