About this Journal Submit a Manuscript Table of Contents
Gastroenterology Research and Practice
Volume 2013 (2013), Article ID 107534, 7 pages
http://dx.doi.org/10.1155/2013/107534
Clinical Study

Expression Profiling of Proliferation and Apoptotic Markers along the Adenoma-Carcinoma Sequence in Familial Adenomatous Polyposis Patients

1Department of Histopathology, Imperial College London, Hammersmith Hospital, DuCane Road, London W12 0HS, UK
2Department of Surgery, Faculty of Medicine, Alexandria University, Alexandria, Egypt
3Department of Surgery, Charing Cross Hospital, London W6 8RF, UK
4Department of Histopathology, St. Mark's Hospital, London HA1 3UJ, UK
5Wellcome Trust Centre for Human Genetics, University of Oxford and NIHR Oxford Biomedical Research Centre, Oxford OX3 7BN, UK
6Centre for Tumour Biology, Barts Cancer Institute, Queen Mary, University of London, London EC1M 6BQ, UK

Received 17 December 2012; Accepted 8 January 2013

Academic Editor: Bita Geramizadeh

Copyright © 2013 Jayson Wang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Introduction. Familial adenomatous polyposis (FAP) patients have a germline mutation in the adenomatous polyposis coli (APC) gene. The APC protein interacts with beta-catenin, resulting in the activation of the Wnt signalling pathway. This results in alterations in cell proliferation and apoptosis. We investigated the expression of beta-catenin and related proliferation and apoptotic factors in FAP patients, exploring the expression along the adenoma-carcinoma sequence. Methods. The expression of beta-catenin, p53, bcl-2, cyclin-D1, caspase-3, CD10, and Ki-67 proteins was studied by immunohistochemistry in samples of colonic nonneoplastic mucosa ( ), adenomas ( ), and adenocarcinomas ( ) from each of the16 FAP patients. Results. The expression of beta-catenin, caspase-3, cyclin-D1, and Ki-67 was increased in both adenomas and carcinomas in FAP patients, compared with normal mucosa. p53 and CD10 expression was only slightly increased in adenomas, but more frequently expressed in carcinomas. Bcl-2 expression was increased in adenomas, but decreased in carcinomas. Conclusion. This is the first study investigating collectively the expression of these molecules together in nonneoplastic mucosa, adenomas, and carcinomas from FAP patients. We find that beta-catenin and related proliferative and apoptotic factors (cyclin-D1, bcl-2, caspase-3, and Ki-67) are expressed early in the sequence, in adenomas. However, p53 and CD10 are often expressed later in the sequence, in carcinomas.