124530.fig.001
Figure 1: Schematic representation of study design. On the day 1, day 15, and day 22, the antigen emulsion was immunized in the paws and groin. On day 29, TNBS-ethanol was instilled into the colon. Clinical observation was performed after 24 hours of TNBS-ethanol enema for 4 consecutive weeks. Rats were weighed every weekend. On the end of 24-hour or 4-week administration, rats were sacrificed, respectively.