ppENK Gene Methylation Status in the Development of Pancreatic Carcinoma

Figure 9

RT-PCR analysis of the 5 pancreatic carcinoma cell lines. Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) serves as an RNA control. The two cancer cell lines do not express ppENK. Treatment of Aspc-1 and Panc-1 with 5-aza-2-deoxycytidine restores the expression of ppENK: lane 1, normal pancreatic tissue (frozen tissue); lane 2, Aspc-1 (before the treatment); lane 3, Aspc-1 (after); lane 4, Panc-1 (before); lane 5, Panc-1 (after); lane 6, negative control.