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Gastroenterology Research and Practice
Volume 2013 (2013), Article ID 951034, 8 pages
http://dx.doi.org/10.1155/2013/951034
Clinical Study

PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients

1Department of Internal Medicine, Faculty of Medical Sciences, State University of Campinas (UNICAMP), 13081-970 Campinas, SP, Brazil
2Nursing Course, Federal University of Mato Grosso do Sul (UFMS), 79620-080 Três Lagoas, MS, Brazil
3Gastrocentro, State University of Campinas (UNICAMP), 13083-878 Campinas, SP, Brazil

Received 23 August 2012; Revised 16 December 2012; Accepted 23 December 2012

Academic Editor: Vikram Kate

Copyright © 2013 Silvia M. Ferreira Menoni et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. D. Y. Graham, H. M. Malaty, D. G. Evans, D. J. Evans, P. D. Klein, and E. Adam, “Epidemiology of Helicobacter pylori in an asymptomatic population in the United States: effect of age, race, and socioeconomic status,” Gastroenterology, vol. 100, no. 6, pp. 1495–1501, 1991. View at Scopus
  2. J. M. Scheiman and A. F. Cutler, “Helicobacter pylori and gastric cancer,” American Journal of Medicine, vol. 106, no. 2, pp. 222–226, 1999. View at Publisher · View at Google Scholar · View at Scopus
  3. C. P. Dooley, H. Cohen, P. L. Fitzgibbons et al., “Prevalence of Helicobacter pylori infection and histologic gastritis in asymptomatic persons,” The New England Journal of Medicine, vol. 321, no. 23, pp. 1562–1566, 1989. View at Scopus
  4. N. S. Taylor, J. G. Fox, N. S. Akopyants et al., “Long-term colonization with single and multiple strains of Helicobacter pylori assessed by DNA fingerprinting,” Journal of Clinical Microbiology, vol. 33, no. 4, pp. 918–923, 1995. View at Scopus
  5. F. Megraud, “Epidemiology of Helicobacter pylori infection,” Gastroenterology Clinics of North America, vol. 22, no. 1, pp. 73–88, 1993.
  6. J. C. Atherton, “The clinical relevance of strain types of Helicobacter pylori,” Gut, vol. 40, no. 6, pp. 701–703, 1997. View at Scopus
  7. M. Radić, D. M. Kaliterna, and J. Radić, “Helicobacter pylori infection and systemic sclerosis-is there a link?” Joint Bone Spine, vol. 78, no. 4, pp. 337–340, 2011. View at Publisher · View at Google Scholar
  8. V. Bassi, C. Santinelli, A. Iengo, and C. Romano, “Identification of a Correlation between Helicobacter pylori Infection and Graves' Disease,” Helicobacter, vol. 15, no. 6, pp. 558–562, 2010. View at Publisher · View at Google Scholar · View at Scopus
  9. R. Jesnowski, B. Isaksson, C. Möhrcke et al., “Helicobacter pylori in autoimmune pancreatitis and pancreatic carcinoma,” Pancreatology, vol. 10, no. 4, pp. 462–466, 2010. View at Publisher · View at Google Scholar
  10. M. F. Go and D. Y. Graham, “How does Helicobacter pylori cause duodenal ulcer disease: the bug, the host, or both?” Journal of Gastroenterology and Hepatology, vol. 9, supplement 1, pp. S8–S10, 1994. View at Scopus
  11. A. Labigne and H. De Reuse, “Determinants of Helicobacter pylori pathogenicity,” Infectious Agents and Disease, vol. 5, no. 4, pp. 191–202, 1996. View at Scopus
  12. B. E. Dunn, H. Cohen, and M. J. Blaser, “Helicobacter pylori,” Clinical Microbiology Reviews, vol. 10, no. 4, pp. 720–741, 1997. View at Scopus
  13. L. G. Davis, M. D. Dibner, and J. F. Battey, Basic Metthods in Molecular Biology, Elsevier, New York, NY, USA, 1995.
  14. D. S. Latchman, PCR Applications in Pathology. Principles and Practice, Oxford University Press, 1995.
  15. R. K. Saiki, S. Scharf, and F. Faloona, “Enzymatic amplification of β-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia,” Science, vol. 230, no. 4732, pp. 1350–1354, 1985. View at Scopus
  16. C. Li, T. Ha, D. S. Chi et al., “Differentiation of Helicobacter pylori strains directly from gastric biopsy specimens by PCR-based restriction fragment length polymorphism analysis without culture,” Journal of Clinical Microbiology, vol. 35, no. 12, pp. 3021–3025, 1997.
  17. E. Kague, C. M. Thomazini, M. I. D. C. M. Pardini, F. De Carvalho, C. V. Leite, and N. A. Pinheiro, “Methylation status of CDH1 gene in samples of gastric mucous from Brazilian patients with chronic gastritis infected by Helicobacter pylori,” Arquivos de Gastroenterologia, vol. 47, no. 1, pp. 7–12, 2010. View at Publisher · View at Google Scholar · View at Scopus
  18. N. Dorrell, J. E. Crabtree, and B. W. Wren, “Host-bacterial interactions and the pathogenesis of Helicobacter pylori infection,” Trends in Microbiology, vol. 6, no. 10, pp. 379–380, 1998. View at Publisher · View at Google Scholar · View at Scopus
  19. N. C. Quaglia, A. Dambrosio, G. Normanno, and G. V. Celano, “Evaluation of a Nested-PCR assay based on the phosphoglucosamine mutase gene (glmM) for the detection of Helicobacter pylori from raw milk,” Food Control, vol. 20, no. 2, pp. 119–123, 2009. View at Publisher · View at Google Scholar · View at Scopus
  20. L.-J. Van Doorn, C. Figueiredo, R. Sanna et al., “Expanding allelic diversity of Helicobacter pylori vacA,” Journal of Clinical Microbiology, vol. 36, no. 9, pp. 2597–2603, 1998.
  21. A. A. Van Zwet, J. C. Thijs, A. M. D. Kooistra-Smid, J. Schirm, and J. A. M. Snijder, “Sensitivity of culture compared with that of polymerase chain reaction for detection of Helicobacter pylori from antral biopsy samples,” Journal of Clinical Microbiology, vol. 31, no. 7, pp. 1918–1920, 1993. View at Scopus
  22. L. Engstrand, C. Pahlson, S. Gustavsson, and A. Schwan, “Monoclonal antibodies for rapid identification of Campylobacter pyloridis,” The Lancet, vol. 2, no. 8520, pp. 1402–1403, 1986. View at Scopus
  23. H. Lamouliatte, J. Hua, C. Birac, R. Caular, and F. Mégraud, “Posttreatment follow-up of anti-Helicobacter pylori regimen: standard bacteriology or PCR?” Acta Gastro-Enterologica Belgica, vol. 56, supplement, pp. 104–119, 1993.
  24. F. Mégraud, “Advantages and disadvantages of current diagnostic tests for the detection of Helicobacter pylori,” Scandinavian Journal of Gastroenterology, Supplement, vol. 31, no. 215, pp. 57–62, 1996.
  25. R. P. H. Logan and D. E. Berg, “Genetic diversity of Helicobacter pylori,” The Lancet, vol. 348, no. 9040, pp. 1462–1463, 1996. View at Scopus
  26. T. L. Cover, “The vacuolating cytotoxin of Helicobacter pylori,” Molecular Microbiology, vol. 20, no. 2, pp. 241–246, 1996. View at Publisher · View at Google Scholar · View at Scopus
  27. K. J. Forbes, Z. Fang, and T. H. Pennington, “Allelic variation in the Helicobacter pyloriflagellin genes flaA and flaB: its consequences for strain typing schemes and population structure,” Epidemiology and Infection, vol. 114, no. 2, pp. 257–266, 1995. View at Scopus
  28. N. Akopyanz, N. O. Bukanov, T. U. Westblom, and D. E. Berg, “PCR-based RFLP analysis of DNA sequence diversity in the gastric pathogen Helicobacter pylori,” Nucleic Acids Research, vol. 20, no. 23, pp. 6221–6225, 1992. View at Scopus
  29. D. E. Taylor, M. Eaton, N. Chang, and S. M. Salama, “Construction of a Helicobacter pylori genome map and demonstration of diversity at the genome level,” Journal of Bacteriology, vol. 174, no. 21, pp. 6800–6806, 1992. View at Scopus
  30. N. Akopyanz, N. O. Bukanov, T. U. Westblom, S. Kresovich, and D. E. Berg, “DNA diversity among clinical isolates of Helicobacter pylori detected by PCR-based RAPD fingerprinting,” Nucleic Acids Research, vol. 20, no. 19, pp. 5137–5142, 1992. View at Scopus
  31. M. Desai, D. Linton, R. J. Owen, and J. Stanley, “Molecular typing of Helicobacter pylori isolates from asymptomatic, ulcer and gastritis patients by urease gene polymorphism,” Epidemiology and Infection, vol. 112, no. 1, pp. 151–160, 1994. View at Scopus
  32. M. J. Blaser, “Intrastrain differences in Helicobacter pylori: a key question in mucosal damage?” Annals of Medicine, vol. 27, no. 5, pp. 559–563, 1995. View at Scopus
  33. “NIH Consensus Conference. Helicobacter pylori in peptic ulcer disease. NIH Consensus Development Panel on Helicobacter pylori in Peptic Ulcer Disease,” JAMA, vol. 272, no. 1, pp. 65–69, 1994.
  34. C. L. Clayton, H. Kleanthous, D. D. Morgan, L. Puckey, and S. Tabaqchali, “Rapid fingerprinting of Helicobacter pylori by polymerase chain reaction and restriction fragment length polymorphism analysis,” Journal of Clinical Microbiology, vol. 31, no. 6, pp. 1420–1425, 1993. View at Scopus
  35. P. A. Foxall, L. T. Hu, and H. L. T. Mobley, “Use of polymerase chain reaction-amplified Helicobacter pylori urease structural genes for differentiation of isolates,” Journal of Clinical Microbiology, vol. 30, no. 3, pp. 739–741, 1992. View at Scopus
  36. S. Fujimoto, B. Marshall, and M. J. Blaser, “PCR-based restriction fragment length polymorphism typing of Helicobacter pylori,” Journal of Clinical Microbiology, vol. 32, no. 2, pp. 331–334, 1994. View at Scopus
  37. C. R. Lopez, R. J. Owen, and M. Desai, “Differentiation between isolates of Helicobacter pylori by PCR-RFLP analysis of urease A and B genes and comparison with ribosomal RNA gene patterns,” FEMS Microbiology Letters, vol. 110, no. 1, pp. 37–44, 1993. View at Publisher · View at Google Scholar · View at Scopus
  38. R. A. Moore, A. Kureishi, S. Wong, and L. E. Bryan, “Categorization of clinical isolates of Helicobacter pylori on the basis of restriction digest analyses of polymerase chain reaction-amplified ureC genes,” Journal of Clinical Microbiology, vol. 31, no. 5, pp. 1334–1335, 1993. View at Scopus