PCR-Based Detection and Genotyping of Helicobacter pylori in Endoscopic Biopsy Samples from Brazilian Patients

Table 4

Use of the Restriction Fragment Length Products (RFLP) technique for genotyping the positive H. pylori PCR products using restriction enzymes (HhaI and MboI).

Restriction enzyme	Frequency (%)	Disease	Median age (years)
HhaI-1	1/58 ( 1.7)	Gastritis + ulcers	41
HhaI-2	5/58 (8.6)	3 Gastritis; 2 ulcers	29
HhaI-3	12/58 (20.7)	9 Gastritis; 3 ulcers;	45
HhaI-4	14/58 (24.1)	8 Gastritis; 3 ulcers; 1 esophagitis; 1 Inflamation	39
HhaI-5	3/58 (5.2)	1 Gastritis; 2 ulcers	54
HhaI-6	6/58 (10.3)	3 Gastritis; 2 ulcers; 1 Gastritis + ulcers	39
HhaI-7	1/58 (1.7)	1 ulcers	19
HhaI-8	4/58 (6.9)	1 Gastritis; 2 ulcers; 1 esophagitis	48
HhaI-9	2/58 (3.4)	1 Gastritis; 1 ulcers	37
HhaI-10	2/58 (3.4)	2 ulcers	72
HhaI-11	8/58 (13.8)	6 Gastritis; 1 inflamation; 1 ulcers	54
MboI-1	2/58 (3.4)	2 Gastritis	72
MboI-2	13/58 (22.4)	7 Gastritis; 5 ulcers, 1 esophagitis	39
MboI-3	8/58 (13.8)	3 Gastritis; 3 ulcers; 1 inflamation	49
MboI-4	15/58 (25.8)	10 Gastritis; 5 ulcers	57
MboI-5	4/58 (6.9)	1 Gastritis; 1 ulcers; 1 esophagitis; 1 Gastritis ulcers	27
MboI-6	2/58 (3.4)	1 Gastritis; 1 ulcers	37
MboI-7	4/58 (6.9)	1 Gastritis, 1 splenomegaly; 2 ulcers	50
MboI-8	1/58 (1.7)	1 Gastritis	45
MboI-9	5/58 (8.6)	2 Gastritis; 2 ulcers; 1 Gastritis + ulcers	47
MboI-10	2/58 (3.4)	2 Gastritis	28
MboI-11	1/58 (1.7)	1 Gastritis	31
MboI-12	1/58 (1.7)	1 Gastritis	38