Overexpression of FOXO3, MYD88, and GAPDH Identified by Suppression Subtractive Hybridization in Esophageal Cancer Is Associated with Autophagy

Table 1

Designed primers sequences used to quantify gene expression by real-time PCR.

Application	Primer name	Accession number	Length	Sequence (5′ to 3′)	Annealing temperature	Location	Product size
Overexpressed genes	FOXO3-F	NM_001455.3	20	ACGGTGTTCGGACCTTCATC	60°C	1613–1632	196 bp
	FOXO3-R	NM_001455.3	20	TGCTGGCCTGAGACATCAAG		1789–1808
	GAPDH-F	NM_002046.4	20	CGACAGTCAGCCGCATCTTC	61°C	118–137	250 bp
	GAPDH-R	NM_002046.4	20	CGTTCTCAGCCTTGACGGTG		348–367
	MYD88-v1-F	NM_001172567.1	20	TCATCGAAAAGAGGTTGGCT	57°C	855–875	158 bp
	MYD88-v1-R	NM_001172567.1	20	GATGGGGATCAGTCGCTTCT		993–1012
	MYD88-v2-F	NM_002468.4	20	CCACACTTGATGACCCCCTG	61°C	666–685	210 bp
	MYD88-v2-R	NM_002468.4	20	GGCGGCACCTCTTTTCGATG		856–875
	MYD88-v3-F	NM_001172568.1	20	GGGACCCAGCATTGGGCATA	61°C	538–557	289 bp
	MYD88-v3-R	NM_001172568.1	20	ACCTGGAGAGAGGCTGAGTG		807–826
	MYD88-v4-F	NM_001172569.1	20	CTTGATGACCCCCTGGGTGC	62°C	671–690	207 bp
	MYD88-v4-R	NM_001172569.1	20	GGTTGGTGTAGTCGCAGACA		858–877
	MYD88-v5-F	NM_001172566.1	17	ACCCAGCATTGGTGCCG	60°C	541–557	202 bp
	MYD88-v5-R	NM_001172566.1	20	GGTTGGTGTAGTCGCAGACA		723–742
Control gene	ACTB-F	NM_001101.3	21	ATGGCCACGGCTGCTTCCAGC	60°C	763–783	322 bp
	ACTB-R	NM_001101.3	21	CAGGAGGAGCAATGATCTTGA		1064–1084
Autophagy-related genes	Gabarapl1-F	NM_031412.2	20	CAGGGTCCCCGTGATTGTAG	61°C	321–340	179 bp
	Gabarapl1-R	NM_031412.2	20	TGGGAGGGATGGTGTTGTTG		480–499
	Beclin1-F	NM_003766.3	20	GCTGAAGACAGAGCGATGGT	59.5°C	30–49	169 bp
	Beclin1-R	NM_003766.3	20	CATGGTGCTGTTGTTGGACG		179–198
	PIK3C3-F	NM_002647.2	20	GGCACACAGAGTGAGCAGTA	59.5°C	2423–2442	204 bp
	PIK3C3-R	NM_002647.2	20	CACAGCCTCTTCATCCGACA		2607–2626
	ATG12-F	NM_004707.3	20	TGCTGGAGGGGAAGGACTTA	59.5°C	347–366	186 bp
	ATG12-R	NM_004707.3	20	GTTCGCTCTACTGCCCACTT		513–532
	MAP1LC3B-F	NM_022818.4	20	GCCGCCTTTTTGGGTAGAAG	59.5°C	1012–1031	225 bp
	MAP1LC3B-F	NM_022818.4	20	TACCCCTGCAAGAGTGAGGA		1217–1236